Reference - Detail
| RRC ID | 48574 |
|---|---|
| Author | Cui Y, Zhao Q, Xie HT, Wong WS, Wang X, Gao C, Ding Y, Tan Y, Ueda T, Zhang Y, Jiang L. |
| Title | MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development. |
| Journal | Plant Physiol |
| Abstract |
Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating tapetal PCD Here, we demonstrate that Rab7-mediated vacuolar transport of tapetum degradation-related cysteine proteases is crucial for tapetal PCD and pollen development in Arabidopsis (Arabidopsis thaliana), with the following evidence: (1) The monensin sensitivity1 (mon1) mutants, which are defective in Rab7 activation, showed impaired male fertility due to a combined defect in both tapetum and male gametophyte development. (2) In anthers, MON1 showed preferential high level expression in tapetal cell layers and pollen. (3) The mon1 mutants exhibited delayed tapetum degeneration and tapetal PCD, resulting in abnormal pollen coat formation and decreased male fertility. (4) MON1/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-mediated Rab7 activation was indispensable for vacuolar trafficking of tapetum degradation-related cysteine proteases, supporting that PCD-triggered tapetum degeneration requires Rab7-mediated vacuolar trafficking of these cysteine proteases. (5) MON1 mutations also resulted in defective pollen germination and tube growth. Taken together, tapetal PCD and pollen development require successful MON1/CCZ1-mediated vacuolar transport in Arabidopsis. |
| Volume | 173(1) |
| Pages | 206-218 |
| Published | 2017-1-1 |
| DOI | 10.1104/pp.16.00988 |
| PII | pp.16.00988 |
| PMID | 27799422 |
| PMC | PMC5210713 |
| MeSH | Apoptosis* Arabidopsis / cytology* Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Cysteine Proteases / metabolism Enzyme Activation Genes, Dominant Germination Guanine Nucleotide Exchange Factors / metabolism* Models, Biological Mutation / genetics Plant Infertility / genetics Plant Leaves / metabolism Pollen / cytology* Pollen / metabolism* Pollen / ultrastructure Pollen Tube / growth & development Pollen Tube / metabolism Protoplasts / metabolism Vacuoles / metabolism Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / metabolism* rab7 GTP-Binding Proteins |
| IF | 6.902 |
| Times Cited | 9 |
| WOS Category | PLANT SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst05666 |