Reference - Detail
RRC ID | 48799 |
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Author | Wei Y, Diao LX, Lu S, Wang HT, Suo F, Dong MQ, Du LL. |
Title | SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage. |
Journal | Mol Cell |
Abstract |
The action of DNA topoisomerase II (Top2) creates transient DNA breaks that are normally concealed inside Top2-DNA covalent complexes. Top2 poisons, including ubiquitously present natural compounds and clinically used anti-cancer drugs, trap Top2-DNA complexes. Here, we show that cells actively prevent Top2 degradation to avoid the exposure of concealed DNA breaks. A genome-wide screen revealed that fission yeast cells lacking Rrp2, an Snf2-family DNA translocase, are strongly sensitive to Top2 poisons. Loss of Rrp2 enhances SUMOylation-dependent ubiquitination and degradation of Top2, which in turn increases DNA damage at sites where Top2-DNA complexes are trapped. Rrp2 possesses SUMO-binding ability and prevents excessive Top2 degradation by competing against the SUMO-targeted ubiquitin ligase (STUbL) for SUMO chain binding and by displacing SUMOylated Top2 from DNA. The budding yeast homolog of Rrp2, Uls1, plays a similar role, indicating that this genome protection mechanism is widely employed, a finding with implications for cancer treatment. |
Volume | 66(5) |
Pages | 581-596.e6 |
Published | 2017-6-1 |
DOI | 10.1016/j.molcel.2017.04.017 |
PII | S1097-2765(17)30273-3 |
PMID | 28552615 |
MeSH | DNA Damage* / drug effects DNA Helicases / genetics DNA Helicases / metabolism DNA Topoisomerases, Type II / genetics DNA Topoisomerases, Type II / metabolism* DNA, Fungal / drug effects DNA, Fungal / genetics DNA, Fungal / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Drug Resistance Etoposide / pharmacology Genome, Fungal* / drug effects Genomic Instability* / drug effects Mutation Protein Binding Protein Interaction Domains and Motifs Proteolysis Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Schizosaccharomyces / drug effects Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sumoylation* Topoisomerase II Inhibitors / pharmacology Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism Ubiquitination |
IF | 15.584 |
Times Cited | 10 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
Resource | |
DNA material | pHIS3K-Ptub1 (RDB06564) |