RRC ID 51395
Author Liu H, Dowdle JA, Khurshid S, Sullivan NJ, Bertos N, Rambani K, Mair M, Daniel P, Wheeler E, Tang X, Toth K, Lause M, Harrigan ME, Eiring K, Sullivan C, Sullivan MJ, Chang SW, Srivastava S, Conway JS, Kladney R, McElroy J, Bae S, Lu Y, Tofigh A, Saleh SMI, Fernandez SA, Parvin JD, Coppola V, Macrae ER, Majumder S, Shapiro CL, Yee LD, Ramaswamy B, Hallett M, Ostrowski MC, Park M, Chamberlin HM, Leone G.
Title Discovery of Stromal Regulatory Networks that Suppress Ras-Sensitized Epithelial Cell Proliferation.
Journal Dev Cell
Abstract Mesodermal cells signal to neighboring epithelial cells to modulate their proliferation in both normal and disease states. We adapted a Caenorhabditis elegans organogenesis model to enable a genome-wide mesodermal-specific RNAi screen and discovered 39 factors in mesodermal cells that suppress the proliferation of adjacent Ras pathway-sensitized epithelial cells. These candidates encode components of protein complexes and signaling pathways that converge on the control of chromatin dynamics, cytoplasmic polyadenylation, and translation. Stromal fibroblast-specific deletion of mouse orthologs of several candidates resulted in the hyper-proliferation of mammary gland epithelium. Furthermore, a 33-gene signature of human orthologs was selectively enriched in the tumor stroma of breast cancer patients, and depletion of these factors from normal human breast fibroblasts increased proliferation of co-cultured breast cancer cells. This cross-species approach identified unanticipated regulatory networks in mesodermal cells with growth-suppressive function, exposing the conserved and selective nature of mesodermal-epithelial communication in development and cancer.
Volume 41(4)
Pages 392-407.e6
Published 2017-5-22
DOI 10.1016/j.devcel.2017.04.024
PII S1534-5807(17)30350-7
PMID 28535374
PMC PMC5508591
MeSH Animals Breast Neoplasms / genetics Breast Neoplasms / pathology Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Cell Lineage Cell Proliferation Epithelial Cells / cytology* Epithelial Cells / metabolism* Female Fibroblasts / metabolism Fibroblasts / pathology Gene Expression Profiling Gene Regulatory Networks* Genome Humans Mammary Glands, Animal / cytology Mesoderm / metabolism Mice Mutation / genetics Nuclear Proteins Organ Specificity Phenotype Protein Kinases Protein Serine-Threonine Kinases / metabolism RNA Interference Signal Transduction / genetics Stromal Cells / cytology Stromal Cells / metabolism ras GTPase-Activating Proteins / metabolism ras Proteins / metabolism*
IF 10.092
Times Cited 10
Resource
C.elegans tm1095