論文 - 詳細
| RRC ID | 53325 |
|---|---|
| 著者 | Ishii T, Funato Y, Hashizume O, Yamazaki D, Hirata Y, Nishiwaki K, Kono N, Arai H, Miki H. |
| タイトル | Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans. |
| ジャーナル | PLoS Genet |
| Abstract |
Mg2+ serves as an essential cofactor for numerous enzymes and its levels are tightly regulated by various Mg2+ transporters. Here, we analyzed Caenorhabditis elegans strains carrying mutations in genes encoding cyclin M (CNNM) Mg2+ transporters. We isolated inactivating mutants for each of the five Caenorhabditis elegans cnnm family genes, cnnm-1 through cnnm-5. cnnm-1; cnnm-3 double mutant worms showed various phenotypes, among which the sterile phenotype was rescued by supplementing the media with Mg2+. This sterility was caused by a gonadogenesis defect with severely attenuated proliferation of germ cells. Using this gonadogenesis defect as an indicator, we performed genome-wide RNAi screening, to search for genes associated with this phenotype. The results revealed that RNAi-mediated inactivation of several genes restores gonad elongation, including aak-2, which encodes the catalytic subunit of AMP-activated protein kinase (AMPK). We then generated triple mutant worms for cnnm-1; cnnm-3; aak-2 and confirmed that the aak-2 mutation also suppressed the defective gonadal elongation in cnnm-1; cnnm-3 mutant worms. AMPK is activated under low-energy conditions and plays a central role in regulating cellular metabolism to adapt to the energy status of cells. Thus, we provide genetic evidence linking Mg2+ homeostasis to energy metabolism via AMPK. |
| 巻・号 | 12(8) |
| ページ | e1006276 |
| 公開日 | 2016-8-1 |
| DOI | 10.1371/journal.pgen.1006276 |
| PII | PGENETICS-D-16-00367 |
| PMID | 27564576 |
| PMC | PMC5001713 |
| MeSH | AMP-Activated Protein Kinases Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cation Transport Proteins / genetics* Cyclins / biosynthesis Cyclins / genetics* Germ Cells / growth & development Germ Cells / metabolism Gonads / growth & development Gonads / metabolism Intestinal Mucosa / growth & development Intestinal Mucosa / metabolism Longevity / genetics* Magnesium / metabolism Mechanistic Target of Rapamycin Complex 1 Multigene Family / genetics Multiprotein Complexes / genetics* Mutation Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism RNA Interference Signal Transduction / genetics TOR Serine-Threonine Kinases / genetics* |
| IF | 5.175 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1944 |