論文 - 詳細
| RRC ID | 5706 |
|---|---|
| 著者 | Takaine M, Numata O, Nakano K. |
| タイトル | Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring. |
| ジャーナル | EMBO J |
| Abstract |
The contractile ring (CR) consists of bundled actin filaments and myosin II; however, the actin-bundling factor remains elusive. We show that the fission yeast Schizosaccharomyces pombe IQGAP Rng2 is involved in the generation of CR F-actin and required for its arrangement into a ring. An N-terminal fragment of Rng2 is necessary for the function of Rng2 and is localized to CR F-actin. In vitro the fragment promotes actin polymerization and forms linear arrays of F-actin, which are resistant to the depolymerization induced by the actin-depolymerizing factor Adf1. Our findings indicate that Rng2 is involved in the generation of CR F-actin and simultaneously bundles the filaments and regulates its dynamics by counteracting the effects of Adf1, thus enabling the reconstruction of CR F-actin bundles, which provides an insight into the physical properties of the building blocks that comprise the CR. |
| 巻・号 | 28(20) |
| ページ | 3117-31 |
| 公開日 | 2009-10-21 |
| DOI | 10.1038/emboj.2009.252 |
| PII | emboj2009252 |
| PMID | 19713940 |
| PMC | PMC2771088 |
| MeSH | Actin Cytoskeleton / metabolism* Actins / metabolism Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Cycle Proteins / physiology* Cytokinesis / genetics Cytokinesis / physiology* Cytoskeleton / metabolism GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism GTPase-Activating Proteins / physiology* Mutation Protein Binding Rabbits Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* Spectrometry, Fluorescence Temperature |
| IF | 9.889 |
| 引用数 | 40 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 酵母 | FY15527 |