RRC ID |
5779
|
著者 |
Lan WT, Chen YS, Yanagida F.
|
タイトル |
Isolation and characterization of lactic acid bacteria from Yan-dong-gua (fermented wax gourd), a traditional fermented food in Taiwan.
|
ジャーナル |
J Biosci Bioeng
|
Abstract |
Yan-dong-gua (fermented wax gourd; a traditional fermented food in Taiwan) samples were collected at five time intervals from a fixed fermenting bucket. Eighty-five cultures of lactic acid bacteria (LAB) were isolated from Yan-dong-gua samples, and the isolates were divided into classes by phenotype and then groups by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Phenotypic and biochemical characteristics identified two bacterial groups (A and B) and showed that the majority of the isolates were heterofermentative LAB. Weissella cibaria was the major LAB found during the initial fermentation of yan-dong-gua. However, it was mostly replaced by W. paramesenteroides after 5 days of fermentation. All isolated LAB were able to grow in MRS broth containing 6% NaCl. Furthermore, the antibacterial activities of the isolates were determined, and five of the isolates showed inhibitory activities against the indicator strain Lactobacillus sakei subsp. sakei JCM 1157(T). These results suggest that W. cibaria and W. paramesenteroides are the main LAB present during the fermentation of yan-dong-gua. This is the first report describing the distribution and varieties of LAB that exist in the yan-dong-gua fermentation process.
|
巻・号 |
108(6)
|
ページ |
484-7
|
公開日 |
2009-12-1
|
DOI |
10.1016/j.jbiosc.2009.06.009
|
PII |
S1389-1723(09)00290-4
|
PMID |
19914580
|
MeSH |
Antibiosis / genetics
Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification
Biodiversity
Cucurbitaceae / microbiology*
DNA, Bacterial / genetics
DNA, Ribosomal / analysis
DNA, Ribosomal / chemistry
Fermentation / genetics
Food Handling
Food Microbiology*
Food Preservation
Lactic Acid / metabolism
Lactobacillales / classification
Lactobacillales / genetics
Lactobacillales / isolation & purification*
Microbial Viability / genetics
Molecular Sequence Data
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Taiwan
|
IF |
2.366
|
引用数 |
23
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM1157 |