Reference - Detail
|Author||Wassmer T, Attar N, Harterink M, van Weering JR, Traer CJ, Oakley J, Goud B, Stephens DJ, Verkade P, Korswagen HC, Cullen PJ.|
|Title||The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network.|
Early endosome-to-trans-Golgi network (TGN) transport is organized by the retromer complex. Consisting of cargo-selective and membrane-bound subcomplexes, retromer coordinates sorting with membrane deformation and carrier formation. Here, we describe four mammalian retromers whose membrane-bound subcomplexes contain specific combinations of the sorting nexins (SNX), SNX1, SNX2, SNX5, and SNX6. We establish that retromer requires a dynamic spatial organization of the endosomal network, which is regulated through association of SNX5/SNX6 with the p150(glued) component of dynactin, an activator of the minus-end directed microtubule motor dynein; an association further defined through genetic studies in C. elegans. Finally, we also establish that the spatial organization of the retromer pathway is mediated through the association of SNX1 with the proposed TGN-localized tether Rab6-interacting protein-1. These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting.
|MeSH||Animals Biological Transport / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Carrier Proteins / classification Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Line Dynactin Complex Dyneins / genetics Dyneins / metabolism* Endosomes / metabolism* Humans Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Multiprotein Complexes / metabolism Phylogeny Protein Isoforms / genetics Protein Isoforms / metabolism* RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sorting Nexins Two-Hybrid System Techniques Vesicular Transport Proteins / classification Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism trans-Golgi Network / metabolism*|
|WOS Category||DEVELOPMENTAL BIOLOGY CELL BIOLOGY|