論文 - 詳細
| RRC ID | 18962 |
|---|---|
| 著者 | Ikai N, Nakazawa N, Hayashi T, Yanagida M. |
| タイトル | The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe. |
| ジャーナル | Open Biol |
| Abstract |
Target of rapamycin complexes (TORCs), which are vital for nutrient utilization, contain a catalytic subunit with the phosphatidyl inositol kinase-related kinase (PIKK) motif. TORC1 is required for cell growth, while the functions of TORC2 are less well understood. We show here that the fission yeast Schizosaccharomyces pombe TORC2 has a cell cycle role through determining the proper timing of Cdc2 Tyr15 dephosphorylation and the cell size under limited glucose, whereas TORC1 restrains mitosis and opposes securin-separase, which are essential for chromosome segregation. These results were obtained using the previously isolated TORC1 mutant tor2-L2048S in the phosphatidyl inositol kinase (PIK) domain and a new TORC2 mutant tor1-L2045D, which harbours a mutation in the same site. While mutated TORC1 and TORC2 displayed diminished kinase activity and FKBP12/Fkh1-dependent rapamycin sensitivity, their phenotypes were nearly opposite in mitosis. Premature mitosis and the G2-M delay occurred in TORC1 and TORC2 mutants, respectively. Surprisingly, separase/cut1-securin/cut2 mutants were rescued by TORC1/tor2-L2048S mutation or rapamycin addition or even Fkh1 deletion, whereas these mutants showed synthetic defect with TORC2/tor1-L2045D. TORC1 and TORC2 coordinate growth, mitosis and cell size control, such as Wee1 and Cdc25 do for the entry into mitosis. |
| 巻・号 | 1(3) |
| ページ | 110007 |
| 公開日 | 2011-11-1 |
| DOI | 10.1098/rsob.110007 |
| PII | rsob110007 |
| PMID | 22645648 |
| PMC | PMC3352084 |
| MeSH | Amino Acid Sequence Amino Acid Substitution Bridged Bicyclo Compounds, Heterocyclic / pharmacology CDC2 Protein Kinase / metabolism Cell Cycle Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Division Endopeptidases / genetics Endopeptidases / metabolism* Genes, Fungal Mitosis Molecular Sequence Data Mutation Phosphatidylinositol 3-Kinases / chemistry Phosphatidylinositol 3-Kinases / genetics Phosphatidylinositol 3-Kinases / metabolism* Protein Kinases / chemistry Protein Kinases / genetics Protein Kinases / metabolism* Protein Subunits Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Securin Separase Sequence Homology, Amino Acid Sirolimus / pharmacology Spindle Apparatus / drug effects Spindle Apparatus / genetics Thiazolidines / pharmacology |
| IF | 4.931 |
| 引用数 | 42 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 酵母 | Dclp1 |