RRC ID 48574
Author Cui Y, Zhao Q, Xie HT, Wong WS, Wang X, Gao C, Ding Y, Tan Y, Ueda T, Zhang Y, Jiang L.
Title MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development.
Journal Plant Physiol
Abstract Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating tapetal PCD Here, we demonstrate that Rab7-mediated vacuolar transport of tapetum degradation-related cysteine proteases is crucial for tapetal PCD and pollen development in Arabidopsis (Arabidopsis thaliana), with the following evidence: (1) The monensin sensitivity1 (mon1) mutants, which are defective in Rab7 activation, showed impaired male fertility due to a combined defect in both tapetum and male gametophyte development. (2) In anthers, MON1 showed preferential high level expression in tapetal cell layers and pollen. (3) The mon1 mutants exhibited delayed tapetum degeneration and tapetal PCD, resulting in abnormal pollen coat formation and decreased male fertility. (4) MON1/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-mediated Rab7 activation was indispensable for vacuolar trafficking of tapetum degradation-related cysteine proteases, supporting that PCD-triggered tapetum degeneration requires Rab7-mediated vacuolar trafficking of these cysteine proteases. (5) MON1 mutations also resulted in defective pollen germination and tube growth. Taken together, tapetal PCD and pollen development require successful MON1/CCZ1-mediated vacuolar transport in Arabidopsis.
Volume 173(1)
Pages 206-218
Published 2017-1-1
DOI 10.1104/pp.16.00988
PII pp.16.00988
PMID 27799422
PMC PMC5210713
MeSH Apoptosis* Arabidopsis / cytology* Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Cysteine Proteases / metabolism Enzyme Activation Genes, Dominant Germination Guanine Nucleotide Exchange Factors / metabolism* Models, Biological Mutation / genetics Plant Infertility / genetics Plant Leaves / metabolism Pollen / cytology* Pollen / metabolism* Pollen / ultrastructure Pollen Tube / growth & development Pollen Tube / metabolism Protoplasts / metabolism Vacuoles / metabolism Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / metabolism* rab7 GTP-Binding Proteins
IF 6.902
Times Cited 9
Arabidopsis / Cultured plant cells, genes pst05666