論文 - 詳細
| RRC ID | 52057 |
|---|---|
| 著者 | Ashida Y, Nakajima-Koyama M, Hirota A, Yamamoto T, Nishida E. |
| タイトル | Activin A in combination with ERK1/2 MAPK pathway inhibition sustains propagation of mouse embryonic stem cells. |
| ジャーナル | Genes Cells |
| Abstract |
The Activin/Nodal/TGF-β signaling pathway plays a major role in maintaining mouse epiblast stem cells (EpiSCs). The EpiSC-maintaining medium, which contains Activin A and bFGF, induces differentiation of mouse embryonic stem cells (ESCs) to EpiSCs. Here, we show that Activin A also has an ability to efficiently propagate ESCs without differentiation to EpiSCs when combined with a MEK inhibitor PD0325901. ESCs cultured in Activin+PD retained high-level expression of naive pluripotency-related transcription factors. Genomewide analysis showed that the gene expression profile of ESCs cultured in Activin+PD resembles that of ESCs cultured in 2i. ESCs cultured in Activin+PD also showed features common to the naive pluripotency of ESCs, including the preferential usage of the Oct4 distal enhancer and the self-renewal response to Wnt pathway activation. Our finding shows a role of Activin/Nodal/TGF-β signaling in stabilizing self-renewal gene regulatory networks in ESCs. |
| 巻・号 | 22(2) |
| ページ | 189-202 |
| 公開日 | 2017-2-1 |
| DOI | 10.1111/gtc.12467 |
| PMID | 28097777 |
| MeSH | Activins / chemistry Activins / pharmacology* Animals Cell Differentiation / physiology Cell Proliferation / physiology Cells, Cultured Germ Layers / cytology Germ Layers / metabolism HEK293 Cells Humans MAP Kinase Signaling System / drug effects* MAP Kinase Signaling System / physiology Mice Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinase Kinases / metabolism Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / drug effects* Mouse Embryonic Stem Cells / metabolism* Pluripotent Stem Cells / cytology Pluripotent Stem Cells / drug effects Pluripotent Stem Cells / metabolism Transcription Factors / metabolism Transforming Growth Factor beta / metabolism Wnt Signaling Pathway |
| IF | 1.655 |
| 引用数 | 5 |
| リソース情報 | |
| ヒト・動物細胞 | E14tg2a(AES0135) |