RRC ID 29303
Author Chen YS, Liou MS, Ji SH, Yu CR, Pan SF, Yanagida F.
Title Isolation and characterization of lactic acid bacteria from yan-tsai-shin (fermented broccoli stems), a traditional fermented food in Taiwan.
Journal J Appl Microbiol
Abstract AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in yan-tsai-shin (fermented broccoli stems), a traditional fermented food in Taiwan.
METHODS AND RESULTS:A total of 226 LAB were isolated; 111 cultures were isolated from samples collected from seven different markets and 115 from six fresh broccoli samples. These isolates were characterized phenotypically and then initially divided into nine groups (r1 to r9) using restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Some isolates were further divided into four additional groups by other genetic analysis. The most common bacterial genera in yan-tsai-shin and fresh broccoli were Weissella, Lactococcus and Lactobacillus. Regional similarities in the LAB, with differences in diversity, were observed in this study. On the basis of phylogenetic analysis of 16S rRNA, rpoA, rpoB and pheS gene sequences, two strains were included in the genera Enterococcus and Lactococcus, respectively, and identified as potential novel species or subspecies. In addition, the novel enterococcal strain, and 33 L. lactis subsp. lactis and two Weissella cibaria strains were found to have bacteriocin-like inhibitory substance (BLIS) producing abilities.
CONCLUSIONS:These results suggest that the LAB play important roles in the fermentation of yan-tsai-shin.
SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first report describing the distribution and varieties of LAB existing in yan-tsai-shin and fresh broccoli. In addition, two potential novel LAB species or subspecies and one potential novel BLIS were also found in this study.
Volume 115(1)
Pages 125-32
Published 2013-7-1
DOI 10.1111/jam.12199
PMID 23565762
MeSH Bacteriocins / metabolism Brassica / microbiology* Fermentation* Food Microbiology Lactobacillales / classification* Lactobacillales / genetics Lactobacillales / isolation & purification Lactobacillus / classification Lactobacillus / genetics Lactobacillus / isolation & purification Lactococcus / genetics Lactococcus / isolation & purification Phylogeny Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S / genetics Taiwan Weissella / classification Weissella / isolation & purification
IF 3.066
Times Cited 14
General Microbes JCM 1157