RRC ID |
3385
|
Author |
Hermann GJ, Schroeder LK, Hieb CA, Kershner AM, Rabbitts BM, Fonarev P, Grant BD, Priess JR.
|
Title |
Genetic analysis of lysosomal trafficking in Caenorhabditis elegans.
|
Journal |
Mol Biol Cell
|
Abstract |
The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.
|
Volume |
16(7)
|
Pages |
3273-88
|
Published |
2005-7-1
|
DOI |
10.1091/mbc.e05-01-0060
|
PII |
E05-01-0060
|
PMID |
15843430
|
PMC |
PMC1165410
|
MeSH |
Acridine Orange / pharmacology
Adaptor Protein Complex 3
Alleles
Amino Acid Sequence
Animals
Biological Transport
Body Size
Caenorhabditis elegans
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / physiology
DNA-Binding Proteins / genetics
Drosophila
Epistasis, Genetic
GTP Phosphohydrolases / metabolism
Genes, Helminth
Genes, Reporter
Green Fluorescent Proteins / metabolism
Guanine Nucleotide Exchange Factors / genetics*
Guanine Nucleotide Exchange Factors / metabolism
Guanine Nucleotide Exchange Factors / physiology
Intestinal Mucosa / metabolism
Lysosomes / metabolism*
Melanosomes / metabolism
Microscopy, Fluorescence
Models, Genetic
Molecular Sequence Data
Mutation
Phenotype
Protein Transport
Sequence Homology, Amino Acid
Temperature
Transcription Factors / genetics
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism*
rab GTP-Binding Proteins / physiology
|
IF |
3.791
|
Times Cited |
149
|
WOS Category
|
CELL BIOLOGY
|
Resource |
C.elegans |
tm920
tm1320
tm584
tm847
tm935
tm933
tm920
tm246 |