論文 - 詳細
RRC ID | 3385 |
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著者 | Hermann GJ, Schroeder LK, Hieb CA, Kershner AM, Rabbitts BM, Fonarev P, Grant BD, Priess JR. |
タイトル | Genetic analysis of lysosomal trafficking in Caenorhabditis elegans. |
ジャーナル | Mol Biol Cell |
Abstract |
The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells. |
巻・号 | 16(7) |
ページ | 3273-88 |
公開日 | 2005-7-1 |
DOI | 10.1091/mbc.e05-01-0060 |
PII | E05-01-0060 |
PMID | 15843430 |
PMC | PMC1165410 |
MeSH | Acridine Orange / pharmacology Adaptor Protein Complex 3 Alleles Amino Acid Sequence Animals Biological Transport Body Size Caenorhabditis elegans Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / physiology DNA-Binding Proteins / genetics Drosophila Epistasis, Genetic GTP Phosphohydrolases / metabolism Genes, Helminth Genes, Reporter Green Fluorescent Proteins / metabolism Guanine Nucleotide Exchange Factors / genetics* Guanine Nucleotide Exchange Factors / metabolism Guanine Nucleotide Exchange Factors / physiology Intestinal Mucosa / metabolism Lysosomes / metabolism* Melanosomes / metabolism Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Mutation Phenotype Protein Transport Sequence Homology, Amino Acid Temperature Transcription Factors / genetics rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism* rab GTP-Binding Proteins / physiology |
IF | 3.791 |
引用数 | 149 |
WOS 分野 | CELL BIOLOGY |
リソース情報 | |
線虫 | tm920 tm1320 tm584 tm847 tm935 tm933 tm920 tm246 |