RRC ID 3385
Author Hermann GJ, Schroeder LK, Hieb CA, Kershner AM, Rabbitts BM, Fonarev P, Grant BD, Priess JR.
Title Genetic analysis of lysosomal trafficking in Caenorhabditis elegans.
Journal Mol Biol Cell
Abstract The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.
Volume 16(7)
Pages 3273-88
Published 2005-7-1
DOI 10.1091/mbc.e05-01-0060
PII E05-01-0060
PMID 15843430
PMC PMC1165410
MeSH Acridine Orange / pharmacology Adaptor Protein Complex 3 Alleles Amino Acid Sequence Animals Biological Transport Body Size Caenorhabditis elegans Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / physiology DNA-Binding Proteins / genetics Drosophila Epistasis, Genetic GTP Phosphohydrolases / metabolism Genes, Helminth Genes, Reporter Green Fluorescent Proteins / metabolism Guanine Nucleotide Exchange Factors / genetics* Guanine Nucleotide Exchange Factors / metabolism Guanine Nucleotide Exchange Factors / physiology Intestinal Mucosa / metabolism Lysosomes / metabolism* Melanosomes / metabolism Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Mutation Phenotype Protein Transport Sequence Homology, Amino Acid Temperature Transcription Factors / genetics rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism* rab GTP-Binding Proteins / physiology
IF 3.791
Times Cited 149
C.elegans tm920 tm1320 tm584 tm847 tm935 tm933 tm920 tm246