論文 - 詳細
| RRC ID | 46099 |
|---|---|
| 著者 | Grill B, Chen L, Tulgren ED, Baker ST, Bienvenut W, Anderson M, Quadroni M, Jin Y, Garner CC. |
| タイトル | RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans. |
| ジャーナル | J Neurosci |
| Abstract |
Previous studies in Caenorhabditis elegans showed that RPM-1 (Regulator of Presynaptic Morphology-1) regulates axon termination and synapse formation. To understand the mechanism of how rpm-1 functions, we have used mass spectrometry to identify RPM-1 binding proteins, and have identified RAE-1 (RNA Export protein-1) as an evolutionarily conserved binding partner. We define a RAE-1 binding region in RPM-1, and show that this binding interaction is conserved and also occurs between Rae1 and the human ortholog of RPM-1 called Pam (protein associated with Myc). rae-1 loss of function causes similar axon and synapse defects, and synergizes genetically with two other RPM-1 binding proteins, GLO-4 and FSN-1. Further, we show that RAE-1 colocalizes with RPM-1 in neurons, and that rae-1 functions downstream of rpm-1. These studies establish a novel postmitotic function for rae-1 in neuronal development. |
| 巻・号 | 32(8) |
| ページ | 2628-36 |
| 公開日 | 2012-2-22 |
| DOI | 10.1523/JNEUROSCI.2901-11.2012 |
| PII | 32/8/2628 |
| PMID | 22357847 |
| PMC | PMC3302171 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Amino Acid Motifs / genetics Amino Acid Sequence / genetics Animals Animals, Genetically Modified Axons / physiology* Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism F-Box Proteins / genetics F-Box Proteins / metabolism Gene Expression Regulation / genetics Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism Humans Immunoprecipitation Luminescent Proteins / genetics Mass Spectrometry Mechanoreceptors / cytology* Microscopy, Confocal Molecular Sequence Data Mutation / genetics Nuclear Matrix-Associated Proteins / deficiency Nuclear Matrix-Associated Proteins / genetics Nuclear Matrix-Associated Proteins / metabolism* Nucleocytoplasmic Transport Proteins / deficiency Nucleocytoplasmic Transport Proteins / genetics Nucleocytoplasmic Transport Proteins / metabolism* Protein Binding / genetics Signal Transduction / genetics Synapses / genetics Synapses / physiology* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism |
| IF | 5.674 |
| 引用数 | 27 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| 線虫 | tm2784 |